MCP-1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86866A
Western blot analysis of A-549 using CCL2/MCP-1 Polyclonal Antibody at 1:2000 dilution.A-549 cells were treated by TNF-α (20 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Western blot analysis of various lysates using CCL2/MCP-1 Polyclonal Antibody at 1:2000 dilution.
Immunohistochemistry of paraffin-embedded Rat spleen using CCL2/MCP-1 Polyclonal antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Mouse spleen using CCL2/MCP-1 Polyclonal antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Human colon carcinoma using CCL2/MCP-1 Polyclonal antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Name
MCP-1 Polyclonal Antibody
Catalog Number
RD86866A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
Immunogen Information
Immunogen
Recombinant fusion protein of human MCP-1
Gene ID
6347
Swissprot
P13500
Synonyms
CCL2GDCF-2HC11HSMCR30MCAFMCP-1MCP1SCYA2SMC-CF
Calculated MW
39kDa
Observed MW
40kDa/55kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas