MDFI Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD81579A
Western blot analysis of extracts of various cell lines using MDFI Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using MDFI Polyclonal Antibody at dilution of 1:3000.
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For more information on our kits, please contact us at info@reddotbiotech.com

Overview

Circle
Product Name
MDFI Polyclonal Antibody
Catalog Number
RD81579A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human MDFI (NP_005577.1).
Gene ID
4188
Swissprot
Q99750
Synonyms
MDFII-MFI-mfa
Calculated MW
25 kDa
Observed MW
35 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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