MDH1 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Western blot analysis of extracts of various cell lines using MDH1 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using MDH1 Polyclonal Antibody at dilution of 1:3000.
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For more information on our kits, please contact us at info@reddotbiotech.com

Overview

Circle
Product Name
MDH1 Polyclonal Antibody
Catalog Number
RD84571A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human MDH1 (NP_005908.1).
Gene ID
4190
Swissprot
P40925
Synonyms
MDH1HEL-S-32MDH-sMDHAMGC:1375MOR2
Calculated MW
27 kDa/36 kDa/38 kDa
Observed MW
36 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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