METTL3 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD85049A
Western blot analysis of extracts of various cell lines using METTL3 Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of C6 cells using METTL3 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using METTL3 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using METTL3 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of Hela cells using METTL3 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U-2 OS cells using METTL3 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
METTL3 Polyclonal Antibody
Catalog Number
RD85049A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.
Immunogen Information
Immunogen
Recombinant fusion protein of human METTL3 (NP_062826.2).
Gene ID
56339
Swissprot
Q86U44
Synonyms
IME4M6AMT-A70Spo8hMETTL3METTL3
Calculated MW
25 kDa/64 kDa
Observed MW
80 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas