MGMT Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD70062A
Western Blot analysis of MCF7 cells using MGMT Polyclonal Antibody at dilution of 1:600
Product Name
MGMT Polyclonal Antibody
Catalog Number
RD70062A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma.
Immunogen Information
Immunogen
Recombinant human Methylated DNA protein cysteine methyltransferase protein
Swissprot
P16455
Synonyms
6 O methylguanine DNA methyltransferase6-O-methylguanine-DNA methyltransferaseAgatAGTAI267024EC 2.1.1.63Methylated DNA protein cysteine methyltransferaseMethylated-DNA--protein-cysteine methyltransferaseMethylguanine DNA methyltransferaseMGC10702
Calculated MW
22 kDa
Observed MW
22 kDa
Applications
Reactivity
Human
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000
Concentration
2 mg/mL
Storage Buffer
PBS with 0.05% Proclin300 and 50% glycerol, pH7.4.
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas