Expression of themidnolingene is developmentally regulated: it is strongly expressed at the mesencephalon (midbrain) of the embryo in day 12.5 (E12.5) mice. Themidnolinencodes a protein of 508 amino acids (aa), which contains a Ubiquitin-like domain. The intracellular distribution of themidnolinwas studied by usingmidnolin-green fluorescent protein (GFP) fusion proteins.Midnolinwas found to be localized in the nucleus and nucleolus, but not in the cytoplasm. The nucleolar localization signal was determined to be a 28aa peptide located at the C-terminal region of themidnolin.May be involved in regulation of genes related to neurogenesis in the nucleolus
Immunogen Information
Immunogen
Synthetic peptide of human MIDN
Swissprot
Q504T8
Synonyms
Midbrain nucleolar proteinmidnMIDNMidnolin
Calculated MW
49 kDa
Gene Accession
NP_796375
Applications
Reactivity
Human,Mouse
Tested Applications
WB,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
0.3 mg/mL
Storage Buffer
PBS with 0.05% sodium azide and 50% glycerol, PH7.4