For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89105A
Western blot analysis of extracts of various cell lines using MIER1 Polyclonal Antibody at1:1000 dilution.
Immunohistochemistry of paraffin-embedded Mouse kidney using MIER1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
This gene encodes a protein that was first identified in Xenopus laevis by its role in a mesoderm induction early response (MIER). The encoded protein functions as a transcriptional regulator. Alternatively spliced transcript variants encode multiple isoforms, some of which lack a C-terminal nuclear localization signal.
Immunogen Information
Immunogen
Recombinant fusion protein of human MIER1
Gene ID
57708
Swissprot
Q8N108
Synonyms
ER1MI-ER1MIER1
Observed MW
8KDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.