For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89122A
Western blot analysis of extracts of various cell lines using MITD1 Polyclonal Antibody at1:1000 dilution.
Immunohistochemistry of paraffin-embedded Human liver using MITD1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Abscission, the separation of daughter cells at the end of cytokinesis, is effected by endosomal sorting complexes required for transport III (ESCRT-III). The protein encoded by this gene functions as a homodimer, with the N-termini binding to a subset of ESCRT-III subunits and the C-termini binding to membranes. The encoded protein regulates ESCRT-III activity and is required for proper cytokinesis. Several transcript variants encoding different isoforms have been found for this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human MITD1
Gene ID
129531
Swissprot
Q8WV92
Synonyms
MITD1
Observed MW
23kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
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