MKL1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD85146A
Western blot analysis of extracts of various cell lines using MKL1 Polyclonal Antibody at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded Human tonsil using MKL1 Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U-2 OS cells using MKL1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Product Name
MKL1 Polyclonal Antibody
Catalog Number
RD85146A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia. Alternative splicing results in multiple transcript variants.
Immunogen Information
Immunogen
Recombinant fusion protein of human MKL1 (NP_065882.1).
Gene ID
57591
Swissprot
Q969V6
Synonyms
MKL1BSACMALMRTF-A
Calculated MW
98 kDa
Observed MW
145 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.