MLLT3/AF9 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Western blot analysis of extracts of various cell lines using MLLT3/AF9 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using MLLT3/AF9 Polyclonal Antibody at 1:1000 dilution.
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Overview

Circle
Product Name
MLLT3/AF9 Polyclonal Antibody
Catalog Number
RD88291A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
Chromatin reader component of the super elongation complex (SEC, a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Specifically recognizes and binds acylated histone H3, with a preference for histone H3 that is crotonylated. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. Recognizes and binds histone H3 crotonylated at 'Lys-9' (H3K9cr, and with slightly lower affinity histone H3 crotonylated at 'Lys-18' (H3K18cr. Also recognizes and binds histone H3 acetylated and butyrylated at 'Lys-9' (H3K9ac and H3K9bu, respectively, but with lower affinity than crotonylated histone H3. In the SEC complex, MLLT3 is required to recruit the complex to crotonylated histones. Recruitment of the SEC complex to crotonylated histones promotes recruitment of DOT1L on active chromatin to deposit histone H3 'Lys-79' methylation (H3K79me. Plays a key role in hematopoietic stem cell (HSC maintenance by preserving, rather than confering, HSC stemness. Acts by binding to the transcription start site of active genes in HSCs and sustaining level of H3K79me2, probably by recruiting DOT1L.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human MLLT3/AF9
Gene ID
4300
Swissprot
P42568
Synonyms
MLLT3AF9YEATS3
Calculated MW
14kDa
Observed MW
15kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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