For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89174A
Western blot analysis of extracts of various cell lines using MLLT4 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded Rat kidney using MLLT4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This gene encodes a multi-domain protein involved in signaling and organization of cell junctions during embryogenesis. It has also been identified as the fusion partner of acute lymphoblastic leukemia (ALL-1) gene, involved in acute myeloid leukemias with t(6;11)(q27;q23) translocation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, however, not all have been fully characterized.[provided by RefSeq, May 2011]
Immunogen Information
Immunogen
Recombinant fusion protein of human MLLT4
Gene ID
4301
Swissprot
P55196
Synonyms
AF6MLL-AF6MLLT4l-afadin
Calculated MW
18kDa/35kDa/84kDa/93kDa/94kDa
Observed MW
110kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.