MRPL2 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Western blot analysis of extracts of various cell lines using MRPL2 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using MRPL2 Polyclonal Antibody at dilution of 1:1000.
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For more information on our kits, please contact us at info@reddotbiotech.com

Overview

Circle
Product Name
MRPL2 Polyclonal Antibody
Catalog Number
RD266280A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL2 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 12q. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human MRPL2 (NP_057034.2).
Gene ID
51069
Swissprot
Q5T653
Synonyms
MRPL2CGI-22MRP-L14RPML14
Calculated MW
33 kDa
Observed MW
33 kDa

Applications

Circle
Reactivity
Human,Mouse
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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