MRPL20 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD218777A
Western blot analysis of RAW264.7 cell lysate  using MRPL20 Polyclonal Antibody at dilution of 1:900
Western blot analysis of RAW264.7 cell lysate using MRPL20 Polyclonal Antibody at dilution of 1:900
Immunohistochemistry of paraffin-embedded Human liver cancer tissue  using MRPL20 Polyclonal Antibody at dilution of 1:60(×200)
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using MRPL20 Polyclonal Antibody at dilution of 1:60(×200)
Immunohistochemistry of paraffin-embedded Human brain tissue  using MRPL20 Polyclonal Antibody at dilution of 1:60(×200)
Immunohistochemistry of paraffin-embedded Human brain tissue using MRPL20 Polyclonal Antibody at dilution of 1:60(×200)
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Overview

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Product Name
MRPL20 Polyclonal Antibody
Catalog Number
RD218777A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit

Background

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MRPL20 is one of more than 70 protein components of mitochondrial ribosomes that are encoded by the nuclear genome. MRPL20 is a subunit of the 39S mitochondrial ribosome. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology.

Immunogen Information

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Immunogen
Fusion protein of human MRPL20
Swissprot
Q9BYC9
Synonyms
39S ribosomal protein L20mitochondrialL20mtMGC4779MGC74465Mitochondrial ribosomal protein L20MRPL 20
Calculated MW
17 kDa
Observed MW
Refer to figures
Gene Accession
BC009515

Applications

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Reactivity
Human, Mouse
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:50-1:200, ELISA 1:5000-1:10000
Concentration
0.96 mg/mL
Storage Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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