MSH4 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD266200A
Western blot analysis of extracts of SKOV3 cells using MSH4 Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of Rat testis using MSH4 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Product Name
MSH4 Polyclonal Antibody
Catalog Number
RD266200A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a member of the DNA mismatch repair mutS family. This member is a meiosis-specific protein that is not involved in DNA mismatch correction, but is required for reciprocal recombination and proper segregation of homologous chromosomes at meiosis I. This protein and MSH5 form a heterodimer which binds uniquely to a Holliday Junction and its developmental progenitor, thus provoking ADP-ATP exchange, and stabilizing the interaction between parental chromosomes during meiosis double-stranded break repair.
Immunogen Information
Immunogen
Recombinant fusion protein of human MSH4 (NP_002431.2).
Gene ID
4438
Swissprot
O15457
Synonyms
MSH4
Calculated MW
104 kDa
Observed MW
105 kDa
Applications
Reactivity
Human,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas