For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90357A
Immunohistochemistry of paraffin-embedded human breast cancer using MST1/STK4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of L929 cells using MST1/STK4 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process.
Immunogen Information
Immunogen
Recombinant fusion protein of human MST1/MST1/MST1/MST1/STK4
Gene ID
6789
Swissprot
Q13043
Synonyms
STK4KRS2MST1YSK3serine/threonine kinase 4
Observed MW
110KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
IHC,IF
Conjugation
Unconjugated
Dilution
IHC 1:50-1:100,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.