For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90228A
Western blot analysis of HepG2 using MT-ND6 Polyclonal Antibody at 1:2000 dilution.
Western blot analysis of various lysates using MT-ND6 Polyclonal Antibody at 1:2000 dilution.
Immunohistochemistry of paraffin-embedded human breast cancer using MT-ND6 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human liver using MT-ND6 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat kidney using MT-ND6 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity and assembly of complex I.
Immunogen Information
Immunogen
A synthetic peptide of human MT-ND6
Gene ID
4541
Swissprot
P03923
Synonyms
MTND6MT-ND6
Calculated MW
76kDa/126kDa
Observed MW
150KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.