MTAP Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86744A
Western blot analysis of extracts of NIH/3T3 cells using MTAP Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using MTAP Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human colon carcinoma using MTAP Polyclonal antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse kidney using MTAP Polyclonal antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat ovary using MTAP Polyclonal antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of HeLa cells using MTAP Polyclonal antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using MTAP Polyclonal antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
MTAP Polyclonal Antibody
Catalog Number
RD86744A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown.
Immunogen Information
Immunogen
Recombinant fusion protein of human MTAP
Gene ID
4507
Swissprot
Q13126
Synonyms
MTAPBDMFDMSFHDMSMFHHEL-249LGMBFMSAPc86fus
Calculated MW
92kDa/97kDa/99kDa
Observed MW
100KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas