Myc-Tag Monoclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD86724A
Western blotting with anti-MYC rabbit monoclonal antibody at dilution of 1:5000.Lane 1: MYC tag transfected HEK 293 cell lysates, lane 2: HEK 293 whole cell lysate
Western blotting with anti-MYC rabbit monoclonal antibody at dilution of 1:5000.Lane 1: MYC tag transfected HEK 293 cell lysates, lane 2: HEK 293 whole cell lysate
Immunofluorescent analysis of 293F cells transfected with Myc-Tag fusion protein, using anti-Myc-Tag rabbit monoclonal antibody at dilution of 1:200.
Immunofluorescent analysis of 293F cells transfected with Myc-Tag fusion protein, using anti-Myc-Tag rabbit monoclonal antibody at dilution of 1:200.
IP Result of 293F cells transfected with Myc-Tag fusion protein,using anti-Myc-Tag rabbit monoclonal antibody . Lane 1:input, lane 2: rabbit IgG Isotype Control, Lane 3: anti-Myc-Tag rabbit monoclonal antibody
IP Result of 293F cells transfected with Myc-Tag fusion protein,using anti-Myc-Tag rabbit monoclonal antibody . Lane 1:input, lane 2: rabbit IgG Isotype Control, Lane 3: anti-Myc-Tag rabbit monoclonal antibody
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For more information on our kits, please contact us at info@reddotbiotech.com

Overview

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Product Name
Myc-Tag Monoclonal Antibody
Catalog Number
RD86724A
Clonality
Monoclonal
Purification Method
Protein A/G purification
Isotype
IgG
Host
Rabbit

Background

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The MYC tag, derived from the c-MYC protein, is a popular epitope tag for detecting the expression of recombinant proteins in yeast, bacteria, insect, and mammalian cell systems. It can be used for affinity chromatography, then used to separate recombin

Immunogen Information

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Immunogen
Synthetic peptide corresponding to Myc tag conjugated to keyhole limpet haemocyanin.
Synonyms
c Mycc Myc Epitope Tagc Myc TagMyc Epitope TagMyc proto-oncogene proteinTranscription factor p64
Calculated MW
45kDa
Observed MW
45kDa

Applications

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Reactivity
All
Tested Applications
WB, IF,IP
Conjugation
Unconjugated
Dilution
WB 1:2000-1:5000, IP 3ug/sample, IF 1:400-800
Concentration
0.2mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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