Myc-Tag Monoclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86723A
Western blotting with anti-MYC Mouse monoclonal antibody at dilution of 1:1000.Lane 1: MYC tag transfected HEK 293 cell lysates, lane 2: HEK 293 whole cell lysate
1x10^6 CHO cells Transfected with a MYC plasmid were stained with 0.2ug Anti-MYC tag mAb(RD86723A) and AF647 conjugated Goat Anti-mouse IgG(H+L);lsotype Control stained with 0.2ug mouse IgG and AF647 conjugated Goat Anti-mouse IgG (H+L)
Product Name
Myc-Tag Monoclonal Antibody
Catalog Number
RD86723A
Clonality
Monoclonal
Purification Method
Protein A/G purification
Isotype
IgG2a
Host
Mouse
Background
The MYC tag, derived from the c-MYC protein, is a popular epitope tag for detecting the expression of recombinant proteins in yeast, bacteria, insect, and mammalian cell systems. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
Immunogen Information
Immunogen
Synthetic peptide corresponding to Myc tag conjugated to keyhole limpet haemocyanin.
Synonyms
c Mycc Myc Epitope Tagc Myc TagMyc Epitope TagMyc proto-oncogene proteinTranscription factor p64
Calculated MW
28kDa
Observed MW
28kDa
Applications
Reactivity
All
Tested Applications
WB,FCM
Conjugation
Unconjugated
Dilution
WB 1:2000-1:4000,FCM:0.2 ug
Concentration
2mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.