N-WASP Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD82701A
Western blot analysis of extracts of various cell lines using N-WASP Polyclonal Antibody at dilution of 1:3000.
Immunohistochemistry of paraffin-embedded Rat brain using N-WASP Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Human colon carcinoma using N-WASP Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse heart using N-WASP Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using N-WASP Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using N-WASP Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L-929 cells using N-WASP Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Product Name
N-WASP Polyclonal Antibody
Catalog Number
RD82701A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a member of the Wiskott-Aldrich syndrome (WAS) protein family. Wiskott-Aldrich syndrome proteins share similar domain structure, and associate with a variety of signaling molecules to alter the actin cytoskeleton. The encoded protein is highly expressed in neural tissues, and interacts with several proteins involved in cytoskeletal organization, including cell division control protein 42 (CDC42) and the actin-related protein-2/3 (ARP2/3) complex. The encoded protein may be involved in the formation of long actin microspikes, and in neurite extension.
Immunogen Information
Immunogen
Recombinant fusion protein of human N-WASP (NP_003932.3).
Gene ID
8976
Swissprot
O00401
Synonyms
WASLN-WASPNWASPWASPB
Calculated MW
54 kDa
Observed MW
65 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:100 IF 1:50-1:100
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas