NADPH oxidase 4 (NOX4) Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD90360A
Western blot analysis of extracts of various cell lines using NADPH oxidase 4 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using NADPH oxidase 4 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat lung using NADPH oxidase 4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat kidney using NADPH oxidase 4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Name
NADPH oxidase 4 (NOX4) Polyclonal Antibody
Catalog Number
RD90360A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.
Immunogen Information
Immunogen
Recombinant fusion protein of human NADPH oxidase 4
Gene ID
50507
Swissprot
Q9NPH5
Synonyms
NOX4KOXKOX-1RENOX
Calculated MW
250kDa
Observed MW
251KDa
Applications
Reactivity
Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:100
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.