NDUFAF2 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD81761A
Western blot analysis of extracts of various cell lines using NDUFAF2 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using NDUFAF2 Polyclonal Antibody at dilution of 1:3000.
Immunofluorescence analysis of L929 cells using NDUFAF2 Polyclonal Antibody at dilution of  1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using NDUFAF2 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Overview

Circle
Product Name
NDUFAF2 Polyclonal Antibody
Catalog Number
RD81761A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene cause progressive encephalopathy resulting from mitochondrial complex I deficiency.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human NDUFAF2 (NP_777549.1).
Gene ID
91942
Swissprot
Q8N183
Synonyms
NDUFAF2B17.2LMMTNNDUFA12Lmimitin
Calculated MW
19 kDa
Observed MW
20 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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