NDUFAF4 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD81772A
Western blot analysis of extracts of various cell lines using NDUFAF4 Polyclonal Antibody at dilution of 1:3000.
Western blot analysis of extracts of various cell lines using NDUFAF4 Polyclonal Antibody at dilution of 1:3000.
Immunofluorescence analysis of HeLa cells using NDUFAF4 Polyclonal Antibody at dilution of  1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using NDUFAF4 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using NDUFAF4 Polyclonal Antibody at dilution of  1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using NDUFAF4 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Overview

Circle
Product Name
NDUFAF4 Polyclonal Antibody
Catalog Number
RD81772A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

Circle
NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene are a cause of mitochondrial complex I deficiency.

Immunogen Information

Circle
Immunogen
Recombinant fusion protein of human NDUFAF4 (NP_054884.1).
Gene ID
29078
Swissprot
Q9P032
Synonyms
NDUFAF4C6orf66HRPAP20HSPC125My013bA22L21.1
Calculated MW
20 kDa
Observed MW
20 kDa

Applications

Circle
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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