NDUFB8 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89282A
Western blot analysis of extracts of various cell lines using NDUFB8 Polyclonal Antibody at 1:500 dilution.
Immunohistochemistry of paraffin-embedded human liver using NDUFB8 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse kidney using NDUFB8 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of A-549 cells using NDUFB8 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using NDUFB8 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using NDUFB8 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Product Name
NDUFB8 Polyclonal Antibody
Catalog Number
RD89282A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I, that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Immunogen Information
Immunogen
Recombinant fusion protein of human NDUFB8
Gene ID
4714
Swissprot
O95169
Synonyms
ASHICI-ASHI
Calculated MW
38kDa/39kDa
Observed MW
39kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.