NEK2 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87681A
Western blot analysis of extracts of HeLa cells using NEK2 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat ovary using NEK2 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Variation
Quantity
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Overview
Product Name
NEK2 Polyclonal Antibody
Catalog Number
RD87681A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene.
Immunogen Information
Immunogen
A synthetic peptide of human NEK2
Gene ID
4751
Swissprot
P51955
Synonyms
NEK2HsPK21NEK2ANLK1PPP1R111RP67
Calculated MW
9kDa
Observed MW
13kDa
Applications
Reactivity
Human,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas