NEK3 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Western blot analysis of extracts of various cell lines using NEK3 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using NEK3 Polyclonal Antibody at 1:1000 dilution.
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Overview

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Product Name
NEK3 Polyclonal Antibody
Catalog Number
RD86855A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

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This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

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Immunogen
Recombinant fusion protein of human NEK3
Gene ID
4752
Swissprot
P51956
Synonyms
NEK3HSPK36
Calculated MW
58kDa/70kDa
Observed MW
70KDa

Applications

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Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Research Areas

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