NELFB Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87383A
Western blot analysis of extracts of various cell lines using NELFB Polyclonal Antibody at 1:3000 dilution.
Variation
Quantity
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Overview
Product Name
NELFB Polyclonal Antibody
Catalog Number
RD87383A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).
Immunogen Information
Immunogen
Recombinant fusion protein of human NELFB
Gene ID
25920
Swissprot
Q8WX92
Synonyms
NELFBCOBRA1NELF-B
Calculated MW
116kDa/119kDa
Observed MW
142kDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.