NELFCD Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89634A
Western blot analysis of extracts of various cell lines using NELFCD Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human colon using NELFCD Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse spleen using NELFCD Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat testis using NELFCD Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Variation
Quantity
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Overview
Product Name
NELFCD Polyclonal Antibody
Catalog Number
RD89634A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The NELF complex of proteins interacts with the DSIF protein complex to repress transcriptional elongation by RNA polymerase II. The protein encoded by this gene is an essential part of the NELF complex. Alternative translation initiation site usage results in the formation of two isoforms with different N-termini.
Immunogen Information
Immunogen
Recombinant fusion protein of human NELFCD
Gene ID
51497
Swissprot
Q8IXH7
Synonyms
NELFCDHSPC130NELF-CNELF-DTH1TH1L
Calculated MW
49kDa
Observed MW
49kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.