NFIB Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD88149A
Western blot analysis of extracts of HeLa cells using NFIB Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat lung using NFIB Polyclonal Antibody at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Variation
Quantity
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Overview
Product Name
NFIB Polyclonal Antibody
Catalog Number
RD88149A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Transcriptional activator of GFAP, essential for proper brain development. Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Immunogen Information
Immunogen
A synthetic peptide of human NFIB
Gene ID
4781
Swissprot
O00712
Synonyms
NFIBCTFHMGIC/NFIBNF-I/BNF1-BNFI-BNFI-REDNFIB2NFIB3
Calculated MW
160kDa/176kDa/179kDa
Observed MW
179kDa
Applications
Reactivity
Human,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:200-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.