NFKBIB Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86846A
Western blot analysis of extracts of various cell lines using NFKBIB Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat lung using NFKBIB Polyclonal Antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human liver cancer using NFKBIB Polyclonal antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human kidney cancer using NFKBIB Polyclonal antibody at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Name
NFKBIB Polyclonal Antibody
Catalog Number
RD86846A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human NFKBIB
Gene ID
4793
Swissprot
Q15653
Synonyms
NFKBIBIKBBTRIP9
Calculated MW
17kDa/21kDa/22kDa/25kDa/32kDa/37kDa
Observed MW
25-80KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.