NFS1 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD253070A
Western blot analysis of HepG2 cell Hela cell Mouse kidney tissue K562 cell lysates  using NFS1 Polyclonal Antibody at dilution of 1:800
Western blot analysis of HepG2 cell Hela cell Mouse kidney tissue K562 cell lysates using NFS1 Polyclonal Antibody at dilution of 1:800
Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue  using NFS1 Polyclonal Antibody at dilution of 1:100(×200)
Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using NFS1 Polyclonal Antibody at dilution of 1:100(×200)
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For more information on our kits, please contact us at info@reddotbiotech.com

Overview

Circle
Product Name
NFS1 Polyclonal Antibody
Catalog Number
RD253070A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit

Background

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Iron-sulfur clusters are required for the function of many cellular enzymes. The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases. Alternatively spliced transcript variants have been described.

Immunogen Information

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Immunogen
Fusion protein of human NFS1
Swissprot
Q9Y697
Synonyms
Cysteine desulfuraseCysteine desulfurasemitochondrialHUSSY 08IscSmitochondrialNFS1NFS1 nitrogen fixation 1NFS1 nitrogen fixation 1 homolog (S. cerevisiae)NFS1NIFSnitrogen fixation 1 (S. cerevisiaehomolog)nitrogen fixation gene 1nitrogen-
Calculated MW
50 kDa
Observed MW
Refer to figures
Gene Accession
BC018471

Applications

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Reactivity
Human, Mouse
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:1000-1:5000, IHC 1:100-1:200, ELISA 1:5000-1:10000
Concentration
1.74 mg/mL
Storage Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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