NFκB-p65 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD77528A
Western Blot analysis of 293T, K562 and Jurkat cell using NFκB-p65 Polyclonal Antibody at dilution of 1:500
Western Blot analysis of 293T, K562 and Jurkat cell using NFκB-p65 Polyclonal Antibody at dilution of 1:500
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Overview

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Product Name
NFκB-p65 Polyclonal Antibody
Catalog Number
RD77528A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

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NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

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Immunogen
Recombinant protein of human RELA
Swissprot
Q04206
Synonyms
Avian reticuloendotheliosis viral (v rel) oncogene homolog AMGC131774NF kappa B p65delta3NFKB3Nuclear Factor NF Kappa B p65 SubunitNuclear factor NF-kappa-B p65 subunitNuclear factor of kappa light polypeptide gene enhancer in B cells 3Nuclear fact
Calculated MW
60 kDa
Gene Accession
BC011603

Applications

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Reactivity
Human,Mouse
Tested Applications
WB,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000
Concentration
0.4 mg/mL
Storage Buffer
PBS with 0.05% sodium azide and 50% glycerol, PH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.

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