NG2/CSPG4 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
Immunohistochemistry of paraffin-embedded Mouse brain using NG2/CSPG4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded Mouse brain using NG2/CSPG4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of A-375 cells using [KO Validated] NG2/CSPG4 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A-375 cells using [KO Validated] NG2/CSPG4 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Overview

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Product Name
NG2/CSPG4 Polyclonal Antibody
Catalog Number
RD90353A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

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A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.

Immunogen Information

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Immunogen
Recombinant fusion protein of human NG2/CSPG4
Gene ID
1464
Swissprot
Q6UVK1
Synonyms
CSPG4HMW-MAAMCSPMCSPGMEL-CSPGMSK16NG2chondroitin sulfate proteoglycan 4
Calculated MW
42kDa
Observed MW
42KDa

Applications

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Reactivity
Human
Tested Applications
IHC,IF
Conjugation
Unconjugated
Dilution
IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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