NGEF Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD252771A
Western blot analysis of Rat brain tissue and Mouse brain tissue lysates using NGEF Polyclonal Antibody at dilution of 1:250
Immunohistochemistry of paraffin-embedded Human tonsil tissue using NGEF Polyclonal Antibody at dilution of 1:35(×200)
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using NGEF Polyclonal Antibody at dilution of 1:35(×200)
Product Name
NGEF Polyclonal Antibody
Catalog Number
RD252771A
Clonality
Polyclonal
Purification Method
Antigen affinity purification
Isotype
IgG
Host
Rabbit
Background
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension (By similarity).
Immunogen Information
Immunogen
Fusion protein of human NGEF
Swissprot
Q8N5V2
Synonyms
ARHGEF27Eph receptor-interacting exchange proteinEph-interacting exchange proteinEPHEXINEphexin-1Guanine nucleotide exchange factorneuronalNeuronal guanine nucleotide exchange factorNgefNGEF
Calculated MW
82 kDa
Observed MW
Refer to figures
Gene Accession
BC031573
Applications
Reactivity
Human, Mouse, Rat
Tested Applications
WB,IHC,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:30-1:150, ELISA 1:5000-1:10000
Concentration
0.6 mg/mL
Storage Buffer
PBS with 0.05% NaN3 and 40% Glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas