NGLY1 Polyclonal Antibody

For reference only. Please follow the manual included in your kit for instructions.

Catalog Number
RD88364A
Western blot analysis of extracts of various cell lines using NGLY1 Polyclonal Antibody at 1:900 dilution.
Western blot analysis of extracts of various cell lines using NGLY1 Polyclonal Antibody at 1:900 dilution.
Immunofluorescence analysis of PC-12 cells using NGLY1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using NGLY1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
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Overview

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Product Name
NGLY1 Polyclonal Antibody
Catalog Number
RD88364A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit

Background

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This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

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Immunogen
Recombinant fusion protein of human NGLY1
Gene ID
55768
Swissprot
Q96IV0
Synonyms
NGLY1CDDGCDG1VPNG1PNGase
Calculated MW
46kDa/60kDa
Observed MW
75kDa

Applications

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Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

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