NKIRAS1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD88031A
Western blot analysis of extracts of various cell lines using NKIRAS1 Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of NIH/3T3 cells using NKIRAS1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using NKIRAS1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
NKIRAS1 Polyclonal Antibody
Catalog Number
RD88031A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and mediating cytoplasmic retention of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB.
Immunogen Information
Immunogen
Recombinant fusion protein of human NKIRAS1
Gene ID
28512
Swissprot
Q9NYS0
Synonyms
NKIRAS1KBRAS1kappaB-Ras1
Calculated MW
10kDa
Observed MW
15kDa
Applications
Reactivity
Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.