NKX6-1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89276A
Western blot analysis of extracts of various cell lines using NKX6-1 Polyclonal Antibody at 1:500 dilution.
Immunohistochemistry of paraffin-embedded mouse pancreas using NKX6-1 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Variation
Quantity
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Overview
Product Name
NKX6-1 Polyclonal Antibody
Catalog Number
RD89276A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
In the pancreas, NKX6.1 is required for the development of beta cells and is a potent bifunctional transcription regulator that binds to AT-rich sequences within the promoter region of target genes Iype et al.
Immunogen Information
Immunogen
A synthetic peptide of human NKX6-1
Gene ID
4825
Swissprot
P78426
Synonyms
NKX6ANKX6.1
Calculated MW
52kDa
Observed MW
53kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.