For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89011A
Western blot analysis of A-549 using NNMT Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human liver using [KO Validated] NNMT Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of human liver cancer using [KO Validated] NNMT Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor.
Immunogen Information
Immunogen
Recombinant fusion protein of human NNMT
Gene ID
4837
Swissprot
P40261
Synonyms
NNMT
Calculated MW
45kDa
Observed MW
46kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
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