NPM1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD240477A
Western Blot analysis of 1)Hela, 2)293T, 3)A549, 4)Jurkat, 5)NIH/3T3, 6)PC-3, 7)Raji, 8)Caco-2 using NPM1 Polyclonal Antibody at dilution of 1:15000
Immunohistochemistry of paraffin-embedded Mouse colon using NPM1 Polycloanl Antibody at dilution of 1:200.
Immunohistochemistry of paraffin-embedded Rat colon using NPM1 Polycloanl Antibody at dilution of 1:200.
Product Name
NPM1 Polyclonal Antibody
Catalog Number
RD240477A
Clonality
Polyclonal
Purification Method
Antigen Affinity Purification
Isotype
IgG
Host
Rabbit
Background
Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective,multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates,dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.
Immunogen Information
Immunogen
Recombinant Mouse Nucleophosmin protein
Gene ID
18148
Swissprot
Q61937
Synonyms
B23MGC104254NO38NPMNPMNPM1Nucleolar phosphoprotein B23Nucleolar protein NO38Nucleophosmin (nucleolar phosphoprotein B23 numatrin)Nucleophosminnucleophosmin nucleoplasmin family member 1Nucleophosmin/nucleoplasmin family member 1Num
Calculated MW
32 kDa
Observed MW
37 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Dilution
WB 1:10000-1:20000 IHC 1:200-1:500
Concentration
1mg/mL
Storage Buffer
PBS with 0.05% Proclin300, 50% glycerol, pH7.3.
Storage Instructions
Store at -20℃. Avoid freeze / thaw cycles.
Research Areas