NRAS Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western blot with NRAS Polyclonal antibody at dilution of 1:1000.lane 1:Jurkat whole cell lysate,lane 2:293T whole cell lysate,lane3：NIH/3T3 whole cell lysate,lane4：PC-12 whole cell lysate,lane5：Rat ovary
Product Name
NRAS Polyclonal Antibody
Catalog Number
RD90677A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein.
Immunogen Information
Immunogen
Recombinant Human NRAS protein expressed by E.coli
Swissprot
P01111
Synonyms
ALPS4AV095280GTPase NRasHRAS1N rasN ras protein part 4Neuroblastoma RAS viral (v ras) oncogene homologNRASNRAS1NS6OTTHUMP00000013879OTTMUSP00000023521RASNTransforming protein N RasTransforming protein N-Rasv ras neuroblastoma RAS viral onco
Calculated MW
63,64,65,69,71,74 kDa
Observed MW
71,74 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000, IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas