NRF2 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western blot analysis of various lysates using NRF2 Polyclonal Antibody at ARC50393-02 dilution.HeLa cells were treated by MG132(50 μM) at 37℃ for 90 minutes.
Western blot analysis of various lysates using NRF2 Polyclonal Antibody at ARC50393-02 dilution.NIH3T3 cells were treated by MG132(50 μM) at 37℃ for 90 minutes.
Western blot analysis of various lysates using NRF2 Polyclonal antibody at dilution.C6 cells were treated by MG132(50 μM) at 37℃ for 90 minutes.
Immunohistochemistry of paraffin-embedded human breast cancer using NRF2 Polyclonal antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human placenta using NRF2 Polyclonal antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Name
NRF2 Polyclonal Antibody
Catalog Number
RD86739A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene.
Immunogen Information
Immunogen
Recombinant fusion protein of human NRF2
Gene ID
4780
Swissprot
Q16236
Synonyms
NFE2L2HEBP1NRF2
Calculated MW
29kDa/79kDa/85kDa/86kDa
Observed MW
87kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.