NUAK1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD267108A
Western blot analysis of extracts of Rat heart using NUAK1 Polyclonal Antibody at dilution of 1:1000.
Product Name
NUAK1 Polyclonal Antibody
Catalog Number
RD267108A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
NUAK family SNF1-like kinase 1also known asAMPK-related protein kinase 5(ARK5) is anenzymethat in humans is encoded by theNUAK1gene. Serine/threonine-protein kinase involved in various processes such as cell adhesion, regulation of cell ploidy and senescence, cell proliferation and tumor progression. Phosphorylates ATM, CASP6, LATS1, PPP1R12A and p53/TP53. Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of 'Ser-464' of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1: phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1.
Immunogen Information
Immunogen
Recombinant fusion protein of human NUAK1 (NP_055655.1).
Gene ID
9891
Swissprot
O60285
Synonyms
NUAK1ARK5
Calculated MW
23 kDa/74 kDa
Observed MW
74 kDa
Applications
Reactivity
Human,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:200-1:2000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas