NUMB Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD85314A
Western blot analysis of extracts of various cell lines using NUMB Polyclonal Antibody at dilution of 1:1000.
Immunofluorescence analysis of L929 cells using NUMB Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Product Name
NUMB Polyclonal Antibody
Catalog Number
RD85314A
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Alternative splicing results in multiple transcript variants.
Immunogen Information
Immunogen
Recombinant fusion protein of human NUMB (NP_003735.3).
Gene ID
8650
Swissprot
P49757
Synonyms
NUMBC14orf41S171c14_5527
Calculated MW
37 kDa/48-70 kDa
Observed MW
71 kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IF 1:50-1:200
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.
Research Areas