OLA1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87349A
Western blot analysis of extracts of various cell lines using OLA1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded human breast cancer using OLA1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human lung cancer using OLA1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH/3T3 cells using OLA1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using OLA1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
For more information on our kits, please contact us at info@reddotbiotech.com
Overview
Product Name
OLA1 Polyclonal Antibody
Catalog Number
RD87349A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a member of the GTPase protein family. The encoded protein interacts with breast cancer-associated gene 1 (BRCA1) and BRCA1-associated RING domain protein (BARD1), and is involved in centrosome regulation. Overexpression of this gene has been observed in multiple types of cancer and may be associated with poor survival. Pseudogenes of this gene have been defined on chromosomes 17 and 22. [provided by RefSeq, Jun 2016]
Immunogen Information
Immunogen
Recombinant fusion protein of human OLA1
Gene ID
29789
Swissprot
Q9NTK5
Synonyms
OLA1DOC45GBP45GTBP9GTPBP9PTD004
Calculated MW
19kDa/58kDa
Observed MW
59kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.