PAK4 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87346A
Western blot analysis of extracts of LNCaP cells using PAK4 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of various cell lines using PAK4 Polyclonal Antibody at 1:1000 dilution.
Immunofluorescence analysis of NIH/3T3 cells using PAK4 Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
Variation
Quantity
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Overview
Product Name
PAK4 Polyclonal Antibody
Catalog Number
RD87346A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Immunogen Information
Immunogen
A synthetic peptide of human PAK4
Gene ID
10298
Swissprot
O96013
Synonyms
PAK4
Calculated MW
36kDa
Observed MW
36kDa
Applications
Reactivity
Human,Mouse
Tested Applications
WB,IF
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Research Areas