PALLD Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD87457A
Western blot analysis of extracts of various cell lines using PALLD Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat ovary using PALLD Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human colon carcinoma using PALLD Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of HeLa cells using PALLD Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using PALLD Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of A-549 cells using 3ug PALLD Polyclonal Antibody.Western blot was performed from the immunoprecipitate using PALLD Polyclonal Antibody at a dilution of 1:1000.
Variation
Quantity
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Overview
Product Name
PALLD Polyclonal Antibody
Catalog Number
RD87457A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This gene encodes a cytoskeletal protein that is required for organizing the actin cytoskeleton. The protein is a component of actin-containing microfilaments, and it is involved in the control of cell shape, adhesion, and contraction. Polymorphisms in this gene are associated with a susceptibility to pancreatic cancer type 1, and also with a risk for myocardial infarction. Alternative splicing results in multiple transcript variants.
Immunogen Information
Immunogen
Recombinant fusion protein of human PALLD
Gene ID
23022
Swissprot
Q8WX93
Synonyms
PALLDCGI-151CGI151MYNPNCA1SIH002palladin
Calculated MW
97kDa/131kDa
Observed MW
112kDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IF,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IP 1:50-1:200,IHC 1:50-1:200,IF 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.