For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD86666A
Western blotting with anti-PBEF polyclonal Antibody at dilution of 1:1000. Lane 1: Raji cell lysates, lane 2: PC-3 cell lysate, lane 3: MCF-7 cell lysate, lane 4: C6 cell lysate.
PBEF (pre-B cell colony-enhancing factor), also known as visfatin and nicotinamide phosphoribosyltransferase, is an approximately 52 kDa member of the NAPRTase family of molecules. It functions both intracellularly and extracellularly, where it participates in NAD synthesis and insulin receptor activation, respectively.
Immunogen Information
Immunogen
Recombinant Human PBEF protein expressed by E.coli.
Gene ID
10135
Swissprot
P43490
Synonyms
NAMPTPBEFPBEF1Visfatin
Calculated MW
291kDa
Observed MW
290kDa
Applications
Reactivity
Human,Rat
Tested Applications
WB
Conjugation
Unconjugated
Dilution
WB 1:500-1:1000
Concentration
0.6mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol,pH 7.4
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.