PCIF1 Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD89432A
Western blot analysis of extracts of various cell lines using PCIF1 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat brain using PCIF1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse spinal cord using PCIF1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 3ug PCIF1 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using PCIF1 Polyclonal Antibody at a dilution of 1:1000.
Product Name
PCIF1 Polyclonal Antibody
Catalog Number
RD89432A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
Cap-specific adenosine methyltransferase that catalyzes formation of N(6,2'-O-dimethyladenosine cap (m6A(m by methylating the adenosine at the second transcribed position of capped mRNAs. Recruited to the early elongation complex of RNA polymerase II (RNAPII via interaction with POLR2A and mediates formation of m6A(m co-transcriptionally.
Immunogen Information
Immunogen
Recombinant fusion protein of human PCIF1
Gene ID
63935
Swissprot
Q9H4Z3
Synonyms
PCIF1C20orf67PPP1R121PDX1 C-terminal inhibiting factor 1
Calculated MW
44kDa/45kDa
Observed MW
44KDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC,IP
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000,IHC 1:50-1:100,IP 1:50-1:200
Concentration
1mg/mL
Storage Buffer
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Storage Instructions
Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.