PCNA Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
RD232521A
Western Blot analysis of COS-7 cells using Proliferating Cell Nuclear Antigen Polyclonal Antibody at dilution of 1:2000.
Product Name
PCNA Polyclonal Antibody
Catalog Number
RD232521A
Clonality
Polyclonal
Purification Method
Affinity purification
Isotype
IgG
Host
Rabbit
Background
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
Immunogen Information
Immunogen
Synthesized peptide derived from the Internal region of human PCNA
Swissprot
P12004
Synonyms
ATLD2cb16CyclinDNA polymerase delta auxiliary proteinetID36690.10fa28e03fb36g03HGCN8729MGC8367Mutagen-sensitive 209 proteinOTTHUMP00000030189OTTHUMP00000030190PCNAPcna/cyclinPCNAPCNARPolymerase delta accessory proteinProliferating cell nu
Calculated MW
29 kDa
Observed MW
36 kDa
Applications
Reactivity
Human,Mouse,Rat,Monkey
Tested Applications
WB,IHC-p,ELISA
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000, IHC 1:100-1:300, ELISA 1:20000
Concentration
1 mg/mL
Storage Buffer
PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.