PCNA Polyclonal Antibody
For reference only. Please follow the manual included in your kit for instructions.
Catalog Number
Western Blot analysis of HepG2, Hela, Raw264.7 Raji and MCF-7 cells using PCNA Polyclonal Antibody at dilution of 1:500
Immunohistochemistry of paraffin-embedded Human colon cancer using PCNA Polycloanl Antibody at dilution of 1:50
Immunohistochemistry of paraffin-embedded Mouse testis using PCNA Polycloanl Antibody at dilution of 1:50
Immunohistochemistry of paraffin-embedded Rat testis using PCNA Polycloanl Antibody at dilution of 1:50
Product Name
PCNA Polyclonal Antibody
Catalog Number
RD240413A
Clonality
Polyclonal
Purification Method
Antigen Affinity Purification
Isotype
IgG
Host
Rabbit
Background
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
Immunogen Information
Immunogen
Recombinant Human Proliferating cell nuclear antigen protein
Gene ID
5111
Swissprot
P12004
Synonyms
ATLD2cb16CyclinDNA polymerase delta auxiliary proteinetID36690.10fa28e03fb36g03HGCN8729MGC8367Mutagen-sensitive 209 proteinOTTHUMP00000030189OTTHUMP00000030190PCNAPcna/cyclinPCNARPolymerase delta accessory protein
Calculated MW
28 kDa
Observed MW
34 lDa
Applications
Reactivity
Human,Mouse,Rat
Tested Applications
WB,IHC
Conjugation
Unconjugated
Dilution
WB 1:500-1:2000 IHC 1:50-1:200
Concentration
0.28 mg/mL
Storage Buffer
PBS with 0.05% Proclin300, 50% glycerol, pH7.3
Storage Instructions
Store at -20°C. Avoid freeze / thaw cycles.